Table 2. Comparison of amino acid length and number of PNGs between RAL-selected and control-passage KP-1 variants.

	Passage no.	Genetic diversity*	Mean ENV 1–474 length (range)†	Mean V1/V2 length (range)	Mean V3 length (range)	Mean V4 length (range)	Mean PNGs (range)
Baseline	0	0.056	472 (461–480)	69 (60–74)	34 (33–34)	30 (29–31)	24 (22–28)
RAL-selected virus	2	0.038	479 (472–480)‡	74 (71–74)‡	34 (33–34)‡	31 (29–31)‡	27 (25–28) ‡
	8	0.0070	480	74	34	31	28 (26–29)
	17	0.0070	480	74	34	31	27 (26–27)§
Passage control	2	0.045	464 (461–466)‡	64 (60–74)‡	34 (33–34)‡	29 (29–31)‡	24 (22–27)‡
	8	0.0070	463 (462–463)	62	34	29	23 (22–23)
	10	0.0080	462 (459–463)	62	34	29	23 (22–23)
	20	0.010	463	62	34	29	23 (22–23)§
P value			<0.0001‡	<0.0001‡	0.91‡	0.0048‡	0.0019‡
							<0.0001§

^*Overall mean distance.

^†Sequence from gp120 SP to the V5 region (aa 1–474).

^‡, § P values were calculated using the homoscedastic t-test between the RAL-selected and the passage-control variants indicated by the same symbols above.