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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: Regen Med. 2010 Sep;5(5):713–724. doi: 10.2217/rme.10.60

Figure 6. Real-time PCR gene expression of human mesenchymal cells exposed to shear stress.

Figure 6

Cells were exposed to 4, 15 and 22 dynes/cm2 for 24 h after 3 and 7 days in culture in basal (hMSC) and osteogenic supplemented (hMSC-Osts) media. The gene expression of BMP-2 (A), BSP (B) and OP (C) was analyzed. They were calculated using the ΔΔ cycle threshold method and all were normalized to GAPDH. Those values were then normalized to hMSCs and hMSC-Osts not exposed to shear stress and mean values ± standard error of the mean plotted as relative values on a log scale. *p < 0.05 relative to no shear stress in the same group, Kruskal–Wallis one-way ANOVA on ranks. BMP: Bone morphogenetic protein; BSP: Bone sialoprotein; hMSC: Human mesenchymal cell; hMSC-Osts: Human mesenchymal cells exposed to osteogenic supplement; OP: Osteopontin.