Table 1.
Oligonucleotide primers used for quantitative RT-PCR of chicken cytokines
| Type | RNA target1 | Primer sequences2 | PCR product size (bp) | Genbank accession no. |
|---|---|---|---|---|
| Reference | GAPDH | F: 5′-GGTGGTGCTAAGCGTGTTAT-3′ | 264 | K01458 |
| R: 5′-ACCTCTGTCATCTCTCCACA-3′ | ||||
| Proinflammatory | IL1β | F: 5′-TCTGGGACCACTGTATGCTCT-3′ | 256 | AF000631 |
| R: 5′-ACACCAGTGGGAAACAGTATCA-3′ | ||||
| Th-1 | IFNγ | F: 5′-AGCTGACGGTGGACCTATTATT-3′ | 259 | Y07922 |
| R: 5′-GGCTTTGCGCTGGATTC-3′ | ||||
| Th-2 | IL10 | F: 5′-CGGGAGCTGAGGGTGAA-3′ | 272 | AJ621614 |
| R: 5′-GTGAAGAAGCGGTGACAGC-3′ | ||||
| Chemokine | CXCLi2 | F: 5′-GGCTTGCTAGGGGAAATGA-3′ | 200 | AJ009800 |
| R: 5′-AGCTGACTCTGACTAGGAAACTGT-3′ |
1
GAPDH = Glyceraldehyde 3-phosphate dehydrogenase; IL = Interleukin; IFN = Interferon.
2
F = Forward primer; R = Reverse primer.