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. 2014 Mar 13;1:103–113. doi: 10.1016/j.ymgmr.2014.02.002

Fig. 1.

Fig. 1

Expression of hDHCR7 mRNA in livers of treated mice. (a) RT-PCR was performed on liver RNA. Lanes 1 and 2 are from mouse A, lanes 3 and 4 are from mouse B etc. In odd numbered lanes primers to the hDHCR7 gene (123 base pair PCR product) were used, and in even numbered lanes primers to the mouse homologue (mDhcr7, 125 base pair product) were used. mDhcr7 was expressed in both control (A, B, C) and AAV2/8–DHCR7 treated (D, E, F) mice. hDHCR7 was expressed only in the treated mice. Qualitatively similar results were seen from all treated and control mice. (b) Levels of hDHCR7 mRNA expression determined by quantitative RT-PCR. Each bar represents the average relative mRNA expression ± SEM. Relative mRNA expression was calculated by dividing the concentration of hDHCR7 mRNA by the concentration of mGAPDH mRNA. The hDHCR7 expression in mice treated with AAV vector was significantly higher than background levels in sham-treated controls (AAV2–DHCR7 Juveniles: P = 0.03; AAV2/8–DHCR7 Juveniles: P < 0.01; AAV2/8–DHCR7 Newborns: P < 0.01).