FIG 5.

Determination of relative efficiencies of virion envelopment of wild-type and mutant viruses in Vero or BD45 cells. The total number of viral genomes in the cytoplasm of infected Vero or BD45 cells at 24 hpi that were protected from DNase I treatment was obtained by qPCR. The total number of intracellular infectious virions was obtained by plaque assay. Ratios reflecting relative efficiencies of envelopment and infectious virion production were obtained by dividing the average number of capsid-protected viral genomes by the number of PFU. Error bars represent standard errors of the means.