TABLE 2.
Mutational primers for generation of recombinant viruses
| Recombinant virus | Primer name | Primer sequencea |
|---|---|---|
| Mut UL42-D207G | MDV055 D207G-f | TTGAAAGCTGAAGGAGGTTTTTATGCCGGAACGATTTGTGgTGTGATAAGTTTTGATATAGtagggataacagggtaatcgattt |
| MDV055 D207G-r | TTGGACCATTGCGCTTCCATCTATATCAAAACTTATCACAcCACAAATCGTTCCGGCATAAgccagtgttacaaccaattaacc | |
| Mut UL46-Q117R | MDV059 Q117R-f | ACACCTGCGGTGGTAAAAGACTACTACACAGACTCGTATCgGCGCTATGTCTGTAAGCGGCtagggataacagggtaatcgattt |
| MDV059 Q117R-r | ATCAACACAAGGTATACGCCGCCGCTTACAGACATAGCGCcGATACGAGTCTGTGTAGTAGgccagtgttacaaccaattaacc | |
| Mut UL5- I682R | MDV017 I682R-f | GATAGTTATGTCGATAATGTGAGTTCGAGAGGATGTGAGAgATTCATAAACAACATGCGAGtagggataacagggtaatcgattt |
| MDV017 I682R-r | AGCAAGGGATAACATTCCCCCTCGCATGTTGTTTATGAATcTCTCACATCCTCTCGAACTCgccagtgttacaaccaattaacc | |
| Revt UL5-R682I | MDV017 revertant R682I-f | GATAGTTATGTCGATAATGTGAGTTCGAGAGGATGTGAGAtATTCATAAACAACATGCGAGtagggataacagggtaatcgattt |
| MDV017 revertant R682I-r | AGCAAGGGATAACATTCCCCCTCGCATGTTGTTTATGAATaTCTCACATCCTCTCGAACTCgccagtgttacaaccaattaacc | |
| LORF2-Promoter | Rep2 LORF2/17359-f | TCTCGCACAATCTATGCAGAAACAGATAATCTAGGGTGTGtGCGGTGCTTTGTACTTCCTAtagggataacagggtaatcgattt |
| Rep2 LORF2/17359-r | TTTTTATAACTGATCCGACGTAGGAAGTACAAAGCACCGCaCACACCCTAGATTATCTGTTgccagtgttacaaccaattaacc | |
| LORF2-Intron | Rep3 LORF2/17545-f | TTTCCGTCGTGAATTTGTACGCCAAATTTTACAACGTGAGaTTATATTGTTTGAAATTTCAtagggataacagggtaatcgattt |
| Rep3 LORF2/17545-r | ACGATAAAGATAATATACATTGAAATTTCAAACAATATAAtCTCACGTTGTAAAATTTGGCgccagtgttacaaccaattaacc |
a
Nucleotides in uppercase denote the portion of the primer designed to target the MDV genome. The desired point mutation to be incorporated is designated by a bold, underlined lowercase letter. Nucleotides in lowercase indicate the portion of the primer annealing to the plasmid template region containing the kanamycin cassette.