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Activation of ERK1/2 and Akt by transient expression of pUL46 homologs. HEK293T cells were transfected with plasmids expressing wild-type PrV-Ka UL46 (lane 2), PrV-ΔUL34Pass UL46 (lane 3), HSV-1 UL46 (lane 4), VZV ORF12 (lane 5), or the empty pcDNA3.1 vector (lane 1) using Lipofectamine 2000. At 48 h posttransfection, cells were harvested, lysed for Western blotting, and analyzed with antisera as indicated on the left.
