Replication kinetics of UL46-mutants. RK13 cells were infected with wild-type PrV-Ka, PrV-ΔUL46gfp, and PrV-ΔUL46w (A), PrV-ΔUL31Pass, PrV-ΔUL31Pass/ΔUL46gfp, PrV-ΔUL31Pass/ΔUL46w, and PrV-ΔUL31Pass/UL46wt (B) or PrV-ΔUL34Pass, PrV-ΔUL34Pass/ΔUL46gfp, PrV-ΔUL34Pass/ΔUL46w, and PrV-ΔUL34Pass/UL46wt (C) at an MOI of 3. At the time points indicated, cells were scraped into the supernatant, and virus progeny titers were determined on RK13 cells. Mean values of three independent assays and the corresponding standard deviations are indicated.