FIG 2.
Deletion of UL133/8 attenuates the effects of UL97 inhibition on viral replication and viral DNA synthesis. Fibroblasts were infected with WT or UL133/8NULL at an MOI of 1 in the presence of 2 μM MBV or 0.1% DMSO carrier alone. (A and B) Replication curves reflecting the production of cell-free virus are shown for WT (A) and UL133/8NULL (B). Numbers between curves indicate fold defects. Error bars, where perceptible, indicate standard deviations. (C) Results at 144 hpi from three independent biological replicates of the experiment shown in panels A and B are plotted side by side. (D) Levels of viral DNA at 96 hpi from DMSO control- and MBV-treated infections are shown as the number of copies of a viral gene, UL123, normalized to the number of copies of the cellular 18S rDNA determined by qPCR in two independent biological replicates. For panels C and D, error bars indicate SEMs. One-way analysis of variance, followed by Tukey's posttest, was used to determine statistical significance. ns, not significant; *, P < 0.05; **, P < 0.01.