FIG 7.
Disruption of both UL135 and UL138, but not UL138 alone, attenuates the effects of UL97 inhibition on viral replication and viral DNA synthesis. Fibroblasts were infected with the indicated viruses at an MOI of 1 and maintained in the presence of MBV at 2 μM or 0.1% DMSO. The cell-free virus titer was determined for the indicated time points, and the resulting replication kinetics curves are shown for WT (A), UL138STOP (B), and 5/8STOP (C). Error bars indicate standard deviations. (D) Viral yield results at 144 hpi from panels A to C are compared side by side. (E) Viral DNA levels at 96 hpi were determined by qPCR in two independent biological replicates. (Left) Absolute levels of viral DNA; (right) results from MBV conditions as a percentage of the amount of viral DNA measured from the DMSO control conditions. For panel E, error bars indicate standard errors of the means, and a one-way analysis of variance was used to test for statistical significance. P values were determined by Tukey's posttest. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.