FIG 10.

Deletion of the MARs of NoV RdRp results in a severe defect in RNA replication. Total cellular RNAs were isolated from BSR-T7/5 cells transfected with either pT7-N1 (WT), pT7-N1Δ12–34 (ΔMAR1), pT7-N1Δ42–64 (ΔMAR2), or pT7-N1Δ12–64 (ΔMAR1 + 2), separated on denaturing gels, and subjected to Northern blot hybridization analysis as described in the legend to Fig. 1. The positive (A) or negative (B) strands of RNA1 and RNA3 were detected as described; as before, rRNAs were stained with ethidium bromide for use as loading controls (C and D, respectively). (E to H) Quantitation of (+) and (-) strands of N1 and N3 relative to WT is shown for N1 (+) (E), N1 (-) (F), N3 (+) (G), and N3 (-) (H). The relative RNA values from three independent experiments are presented as mean values ± standard deviations.