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NoV RdRp expression was detected at early times posttransfection and reached maximal levels by 20 hpt. BSR-T7/5 cells were transfected with pT7-N1-HA, and PNLs were prepared at 4-h intervals between 4 and 24 hpt. Proteins were separated on an SDS-PAGE gel, and the HA-tagged RdRp was detected by immunoblotting with anti-HA antibodies.
