TABLE 2.
Confocal micrograph by expt | Overlapa |
---|---|
Time course (Fig. 3) | |
4 hpt | 0 |
8 hpt | 88 ± 2.6 |
12 hpt | 86 ± 3.3 |
16 hpt | 89 ± 2.9 |
20 hpt | 82 ± 6.1 |
24 hpt | 83 ± 3.1 |
Selective permeabilization (Fig. 4) | |
Saponin alone, MAO, merge | 82 ± 2.1 |
Saponin Alone, COX3, merge | 0 |
Saponin + Triton X-100, COX3, merge | 88 ± 2.9 |
MAR deletion mutants (Fig. 6) | |
WT, merge | 86 ± 2.3 |
Δ12-34, merge | 88 ± 12.4 |
Δ42-64, merge | 85 ± 5.7 |
Δ12-64, merge | 47 ± 4.2 |
BrU labeling (Fig. 9) | |
Mock, merge | 0 |
WT, merge | 94 ± 1.5 |
Δ12-64, merge | 0 |
Overlap was defined as the percent overlap between red (MTR or TR) and green (FITC) pixels (means ± standard deviations), as described in Materials and Methods and in reference 56.