FIG 4.

Distinct capsid-RNA contacts in the BMV virions. (A) Peptides from the BMV capsid that interact with RNA1 or RNA2 and RNA3/4. Mapping was performed with the RCAP assay. Peaks assigned to peptides within the BMV coat protein were based on theoretical tryptic digests that match calculated masses within 0.5 Da. (B) Molecular models of the peptides within the inner surface of the B1 and the B2.3/4 virions found to contact RNA. The structure was from PDB accession number 1JS9 and was retrieved as a half-capsid from VIPERdb (36). Peptides either common between the B1 and B2.3/4 particles or not found in the RCAP assay are gray, and unique peptides found to contact RNA1 or RNA 2.3/4 are red and blue, respectively.