FIG 1.

Rad18 interacts with BPLF1 in vivo. (A) H1299 cells were transfected with FLAG-tagged BPLF1 1-246. Lysates were immunoprecipitated (IP) with anti-FLAG antibody, followed by immunoblotting with FLAG, Rad18, and PCNA antibodies. Whole-cell lysates were also probed to indicate input levels. (B) Reverse immunoprecipitations were performed to confirm BPLF1 interaction with Rad18. H1299 cells were cotransfected with BPLF1 1-246 and Myc-tagged Rad18. Immunoprecipitations were performed with Myc antibody, followed by immunoblot analysis with Myc, FLAG, and HA antibodies. Whole-cell lysates were also probed.