FIG 4.

Expression of BPLF1 increases Rad18 focus formation. Two separate fields of view are shown for each condition (left panels versus right panels [A-C]). (A) H1299 cells transfected with YFP-Rad18 are homogeneously distributed and are localized primarily in the nucleus. (B) H1299 cells coexpressing the inactive BPLF1 C61S and YFP-Rad18 do not affect focus formation. (C) H1299 cells coexpressing FLAG-BPLF1 and YFP-Rad18 show increased nuclear focus formation of Rad18. (D) Quantification of percentages of cells with focus formation in panels A, B, and C. One hundred cells were counted for each sample.