TABLE 1.

qRT-PCR analysis of genomic segment 8 and cellular ELF^a

Sample	RNA concn (ng/μl)	CT value
		Segment 8b	Cellular ELFc
Organ pool,d fish 1	ND	15.0	21.6
Organ pool,d fish 2	ND	36.16	21.3
Clarified ovarian fluid, fish 1e	12.3	29.37	31.34
Clarified ovarian fluid, fish 2	70.6	37.87	>38
Pellet ovarian fluid, fish 1	13.4	21.47	ND
Pellet ovarian fluid, fish 2	102.6	31.34	ND
Whole egg, fish 1	9.8	32.4	26.54
Internal egg fluid, fish 1	2.2	31.55	35.17
Whole egg, fish 2f	4.5	38.16	29.78
Remaining infective viruse (1 h p.i.)	17.8	28.8	29.1
SHK-1 cells (24 h p.i.)	31.1	23.7	26.97
SHK-1 cellsf (24 h p.i.)	1.3	25.18	24.3

^aELF, elongation factor 1 alpha; ND, not done; p.i., postinfection.

^bSee reference 21.

^cSee reference 22.

^dData provided by SERNAPESCA Chile, obtained from two independent, officially recognized diagnostic laboratories.

^eSource for infection of SHK-1 cells.

^fSource for infection of SHK-1 cells.