FIG 1.

Expression of entry glycoproteins from HSV-1 and SaHV-1. (A) Cell surface expression measured by CELISA. CHO cells in a 96-well plate were transfected overnight with plasmids encoding FLAG-tagged gB, FLAG-tagged gD, gL plus FLAG-tagged gH, gH plus FLAG-tagged gL, or empty vector. The “F-” indicates the constructs that were FLAG tagged. The cells were washed and incubated with an anti-FLAG M2 antibody. After extensive washing, cells were fixed and incubated with an anti-mouse secondary antibody for detection. Each bar shows the mean and standard deviation of three independent determinations. Background signals detected after transfection with the vector alone were subtracted from the values. Data for each set of glycoproteins were normalized to the expression level of HSV-1 F-gD or SaHV-1 F-gD. (B) Total protein expression measured by Western blot of cell lysates. CHO cells expressing the constructs above were lysed, and proteins were resolved by SDS-PAGE. FLAG-tagged gH was coexpressed with FLAG-tagged gL. Proteins were transferred to nitrocellulose and probed with rabbit anti-FLAG antibody followed by goat anti-rabbit IgG. gB, gD, gH, and gL migrated to their expected molecular weights (shown [in thousands] at left). V, empty vector.