FIG 7.

The C-terminal StBox of Vps23 is necessary and sufficient for its recruitment to mitochondria by p36 in BY-2 cells. (A) Schematic diagram illustrating the domain organization of Arabidopsis Vps23 based on Spitzer et al. (63). Numbers denote the specific amino acid residues derived from full-length Vps23 (398 residues) that delineate the protein's UEV, CC, and StBox domains, which are colored red, blue, and yellow, respectively, and which are depicted below as lines representing the various Vps23 truncation mutants described in panels B and C. Numbers denote the specific amino acid residues at the N and C termini of each Vps23 mutant. (B and C) Representative epifluorescence micrographs of BY-2 cells (co)transformed with proteins as indicated by panel labels. Names of mutants represent the specific domain(s) derived from Vps23, as illustrated in panel A. The corresponding merged images (B) and the corresponding DIC images of the UEV-GFP-transformed BY-2 cells (C) are also shown.