FIG 8.

The N-terminal region of p36, like Vps28, interacts with the C-terminal StBox of Vps23 in the yeast two-hybrid assay. (A to D) Yeast strains were cotransformed with the indicated pairs of GAL4-binding domain (BD) and GAL4-activating domain (AD) fusion proteins or the corresponding empty BD or AD control plasmids (indicated by a hyphen in the BD or AD column). Serial (1:5) dilutions of cells were spotted onto agar plates containing either low-stringency medium (SD-Leu,Trp) or high-stringency medium (SD-Leu,Trp,His,Ade), where cell growth is dependent on two-hybrid protein interactions. Note that neither full-length Vps23, nor any of the truncation mutants of Vps23, Vps28, and Vps37 autoactivated the two-hybrid reporter gene system on their own.