FIG 6.

HP1α interaction with Sp100A is reduced during Ad infection. H1299 cells were superinfected with wt virus (H5pg4100) at a multiplicity of infection of 20 FFU/cell 8 h after transfection of 2 μg of pFlag-Sp100A mutant and 3 μg of pYFP-HP1α/pYFP-HP1β, as indicated. Cells were harvested at 24 h p.i., prior to preparation of coimmunoprecipitation (IP) samples and whole-cell extracts. Coprecipitated proteins (A) and input levels (B) of whole-cell lysates were detected using pAb GH3 (anti-Sp100), AC-15 (anti-β-actin), and YFP antibody (anti-YFP-HP1α/pYFP-HP1β). Molecular masses in kDa are indicated on the left, and specific proteins are indicated on the right.