Figure 2.
The identification of the hinge region. (A) The torsional angle difference between two models, 3IYF(open form) and 3J03(closed form). Because of the resolution difference between these two models (3IYF has a resolution of 4.8 Å, whereas 3J03 has a resolution of 8.0 Å), there exists substantial amount of noise. However, the significant angle difference in the figure indicates the existence of a hinge point in the intermediate domain. (B) Schematic of the torsion angle. It is defined as the angle between plane α (GLU 179-GLY 180-LYS 181) and plane β (GLY 180 –LYS 181- VAL 182). (C) A cartoon model of the wild type closed structure of Mm-cpn. The black arrow indicates the location of the hinge point (which is also highlighted via the space-filling model).