Table I.
Melting Temperature and Other Related Physical Parameters of Various AFPs
| Species | Isoform/name | Structure | Size (kDa) | Tm (°C) | Cysteines | Ref |
|---|---|---|---|---|---|---|
| Pseudopleuronectes americanus | 17 kDa | α-helix (Type I) | 16.7 | 55 | 0 | 19 |
| AFP9 | α-helix (Type I) | 4.3 | 18 | 0 | 43 | |
| Macrozoarces americanus | HPLC12 (PDB: 1MSI) | Globular (Type III) | 7.4 | 47 | 0 | 13 |
| HPLC12 (PDB: 1HG7) | Globular (Type III) | 7 | 50 | 0 | 20 | |
| HPLC6 | Globular (Type III) | 6.9 | 40 | 0 | 25 | |
| rAFP (HPLC6, recombinant) | Globular (Type III) | 7.2 | 44 | 0 | 25 | |
| Austrolycicthys brachycephalus | AB1 | Globular (Type III) | 6.8 | 43 | 0 | 26 |
| Dendroides Canadensis | DAFP-4 | β-helical | 7.3 | 84 | 14 | 21 |
| Tenebrio Molitor | YL-1 (PDB: 1EZG) | β-helical | 8.4 | 66 | 16 | 22 |
| Rhagium mordax | RmAFP1 | β-helical | 12.5 | 29 | 2 | This work |