Table III.
Extraction Efficiency and IMAC Recovery of Full-Length Human CFTR
| Detergent used for extraction and purification | Detergent concentration during extraction (mM) | Extraction efficiencya (%) | IMAC recoveryb (%) | Overall recovery (%) |
|---|---|---|---|---|
| UDM | 10 | 84 | 54 | 45 |
| DDM | 9.8 | 79 | 51 | 40 |
| DMNG | 5.3 | 74 | 55 | 41 |
| LMNG | 5.0 | 85 | 43 | 37 |
| C12E8 | 9.3 | 73 | 60 | 44 |
| LPC14 | 10.7 | 117 | 19 | 22 |
| LPC16 | 10.1 | 100 | 16 | 16 |
| CHAPS | 8.1 | 52 | 26 | 14 |
| LPG14 | 10.4 | 115 | 28 | 32 |
| LPG16 | 10.1 | 100 | 13 | 13 |
a
Extraction efficiency = total CFTR extracted/total CFTR found in the membrane preparation.
b
IMAC recovery = total CFTR eluted/total CFTR applied.