Table II.

Thermodynamic Unfolding Parameters for Core Packing Substitutes in Full-Length and Δ_NCap PP32

		m-Value	CM
PP32	−7.93 ± 0.18	2.86 ± 0.02	2.77 ± 0.04
PP32 L22A	−3.88 ± 0.05	2.36 ± 0.03	1.64 ± 0.01
PP32 L37A	−3.68 ± 0.15	2.28 ± 0.07	1.62 ± 0.01
PP32 L47A	−2.98 ± 0.17	2.22 ± 0.06	1.34 ± 0.05
PP32 L60A	−3.98 ± 0.10	2.62 ± 0.01	1.52 ± 0.04
PP32 L83A	−4.46 ± 0.02	3.25 ± 0.02	1.37 ± 0.01
PP32 L109A	−4.56 ± 0.08	3.37 ± 0.17	1.35 ± 0.06
PP32 V135G	−4.07 ± 0.19	3.61 ± 0.16	1.13 ± 0.01
ΔNCap PP32	−4.13 ± 0.09	2.37 ± 0.04	1.74 ± 0.02
ΔNCap PP32 L22A	−4.07 ± 0.25	2.34 ± 0.11	1.64 ± 0.03
ΔNCap PP32 L37A	−3.58 ± 0.16	2.23 ± 0.08	1.60 ± 0.03
ΔNCap PP32 L47A	−2.76 ± 0.12	2.01 ± 0.07	1.37 ± 0.04
ΔNCap PP32 L60A	−3.03 ± 0.37	2.14 ± 0.28	1.42 ± 0.04

Values determined from urea-induced denaturation. in kcal·mol⁻¹; m-values in kcal·mol⁻¹·M⁻¹, C_M in M (urea). Parameters for PP32 Δ_NCap L83A, L109A, and V135G could not be determined.