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. 2014 Jun 18;144(8):1306–1313. doi: 10.3945/jn.114.194407

FIGURE 7.

FIGURE 7

Activation-induced shedding of IL-6R and RNA expression in WT and fat-1 mice. sIL-6R was measured in the supernatant fluids from splenic CD4+ T cells activated with anti-CD3 and anti-CD28 for 48 h (A). TAPI was used to inhibit shedding of IL-6R. Data are combined from 2 separate 48-h incubations, n = 8. Il-6r mRNA concentrations were measured by qPCR (B). Data represent means ± SEMs, n = 5–9. Labeled means without a common letter differ, P < 0.05. CD4+, cluster of differentiation 4+; IL-6R, IL-6 receptor; RPMI, Roswell Park Memorial Institute media; sIL-6R, soluble IL-6 receptor; TAPI, TNF-α protease inhibitor; WT, wild-type.