FIG. 4.

Expression of RNASE9 in mouse epididymis. A) Epididymides were dissected from adult Rnase9^+/+ and Rnase9^−/− mice and cut into five segments: 1) initial segment/proximal caput; 2) midcaput; 3) distal caput; 4) corpus; and 5) cauda. B) Soluble protein fractions of these segments were prepared as described in Materials and Methods and subjected to SDS-PAGE followed by immunoblotting with RNASE9 antiserum (top panel). The membrane was then stripped and reprobed using rabbit anti-mouse GAPDH (bottom panel).