Table 1.

Computer-controlled automatic freezing protocol used for neonatal mouse testicular tissue.

Stages	Cooling rates
Start	4 °C
Ramp	−1 °C/min to 0 °C
Hold	0 °C for 5 min
Ramp	−0.5 °C/min to −8 °C
Hold	−8 °C for 15 min
Ramp	−0.5 °C/min to −40 °C
Hold	−40 °C for 10 min
Ramp	−7 °C/min to −80 °C
Liquid nitrogen	−196 °C