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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: Cryobiology. 2013 May 28;67(1):70–75. doi: 10.1016/j.cryobiol.2013.05.004

Table 3.

Survival and testicular cell distribution rates of fresh and cryopreserved neonatal CD-1 mouse testes post-grafting onto Nude NCr mice (mean ± SEM)

Type of cryoprotectant Survival rate (%) No. of damaged seminiferous tubules (%) Spermatogonia (%) Pachytene spermatocyte (%) Round spermatid (%) Elongated spermatid and spermatozoa (%)
Fresh (control) 100 ± 0.0a 3.2 ± 1.7a 86.6 ± 2.6a 84.6 ± 3.1a 73.3 ± 1.9a 46.6 ± 2.7a
DMSO 100 ± 0.0a 14.3 ± 1.2b 71.4 ± 1.6a 50.0 ± 0.9b 39.7 ± 1.4bd 16.2 ± 1.9b
PrOH 83.3 ± 2.1b 13.4 ± 1.5b 87.3 ± 2.3a 66.6 ± 2.9c 54.5 ± 3.2bc 11.1 ± 1.4b
EG 85.6 ± 3.2b 21.9 ± 2.7c 71.8 ± 3.1a 51.1 ±3.1b 28.5 ± 2.6d 0.0 ± 0.0c
Glycerol 58.6 ± 1.9c 10.1 ± 2.0b 66.6 ± 1.9b 53.0 ± 1.7b 33.3 ± 1.6d 14.6 ± 1.8b

Groups with different letters in the same column are significantly different (p < 0.05).

First column is dimethyl sulfoxide (DMSO), propylene glycol (PrOH), ethylene glycol (EG), glycerol, respectively.