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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: Vet Microbiol. 2013 Feb 6;164(0):27–38. doi: 10.1016/j.vetmic.2013.01.039

Appendix B.

Primers used for detection and full length sequencing of RVCs NSP4, VP4 (VP8* and VP5*), VP6 and VP7 genes designed from human and porcine RVC strains

Gene Name Sequence Segment Amplicon (bp)
Diagnostic (partial VP6) VP6F ACAGTATTTCAGCCAGGDTTTC 1095–1116 260
VP6R AGCCACATAGTTCACATTTCATC 1332–1354
NSP4 NSP4-F GGCTTTAAATTTTTCAGATCAC 1–22 613
NSP4-R AGCCWCATGAATTTTTCAYATC 592–613
VP6 5′comVP6-F GCAWTWAAAATCTCATTCACAATGG 3–27 1352
3′comVP6-R AGCCACATAGTTCACATTTCATCC 1331–1354
VP7 5′comVP7-F GCTGTCTGACAAACTGGTC 20–38 1043
3′comVP7-R GCCACATGATCTTGTTTACGC 1042–1062
VP4-1 (VP8*) VP4-17Fdeg GATCRATGGCGTCYTCAC 17–34 1222
VP4-1238R CCTGATGAATGTAATCCWGGAT 1238–1216
VP4-2 (VP5*) VP4-1108F GATTATTGGGACGATTCAG 1108–1126 1179
VP4-2267F AGCCACATTTCAAGCTGGTC 2267–2286