Appendix B.
Primers used for detection and full length sequencing of RVCs NSP4, VP4 (VP8* and VP5*), VP6 and VP7 genes designed from human and porcine RVC strains
| Gene | Name | Sequence | Segment | Amplicon (bp) |
|---|---|---|---|---|
| Diagnostic (partial VP6) | VP6F | ACAGTATTTCAGCCAGGDTTTC | 1095–1116 | 260 |
| VP6R | AGCCACATAGTTCACATTTCATC | 1332–1354 | ||
| NSP4 | NSP4-F | GGCTTTAAATTTTTCAGATCAC | 1–22 | 613 |
| NSP4-R | AGCCWCATGAATTTTTCAYATC | 592–613 | ||
| VP6 | 5′comVP6-F | GCAWTWAAAATCTCATTCACAATGG | 3–27 | 1352 |
| 3′comVP6-R | AGCCACATAGTTCACATTTCATCC | 1331–1354 | ||
| VP7 | 5′comVP7-F | GCTGTCTGACAAACTGGTC | 20–38 | 1043 |
| 3′comVP7-R | GCCACATGATCTTGTTTACGC | 1042–1062 | ||
| VP4-1 (VP8*) | VP4-17Fdeg | GATCRATGGCGTCYTCAC | 17–34 | 1222 |
| VP4-1238R | CCTGATGAATGTAATCCWGGAT | 1238–1216 | ||
| VP4-2 (VP5*) | VP4-1108F | GATTATTGGGACGATTCAG | 1108–1126 | 1179 |
| VP4-2267F | AGCCACATTTCAAGCTGGTC | 2267–2286 |