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. 2014 May 29;289(28):19279–19293. doi: 10.1074/jbc.M114.570044

FIGURE 1.

FIGURE 1.

Wy14643 increases Hilpda expression in liver slices and hepatocytes. A, the top 40 most highly induced genes in mouse liver slices exposed to 20 μm Wy14643 for 24 h. Values are expressed as -fold changes over DMSO-treated slices from the same animal (n = 4). B, amino acid sequence of mouse and human HILPDA. C, Hilpda mRNA levels determined by qPCR in mouse liver slices exposed to 20 μm Wy14643 for 6 h. Samples include the four mice described in A plus two additional mice (n = 6). Shown are Hilpda mRNA levels in mouse hepatocytes (D, n = 6 animals from different strains) and human hepatocytes (E, n = 6) after exposure to Wy14643 (10 and 50 μm, respectively) for 6 h. F, Hilpda mRNA levels in primary hepatocytes of wild-type and Ppara−/− mice incubated with Wy14643 (10 μm) for 24 h. G, Hilpda mRNA levels in rat hepatocytes incubated with various nuclear receptor ligands for 24 h (RXR agonist LG1069, 1 μm; Wy14643, 5 μm; Fenofibrate, 50 μm; LXR agonist T0901317, 1 μm; FXR agonist chenodeoxycholic acid, 50 μm). H, Hilpda mRNA levels in rat FAO hepatoma cells incubated with Wy14643 (10 μm) for various times. Wy14643 was removed after 4 h of incubation. Data are mean ± S.E. (error bars). Asterisks, significant differences according to Student's t test: *, p < 0.05; **, p < 0.01.