TABLE 2.
Primers used in this study
The abbreviations used are as follows: Fw, forward; Rev, reverse; underlined small letters, restriction sites; italic letters, tags, start, or stop codons.
| Primer | Sequence (5′– 3′) |
|---|---|
| Promotor Fw | aaaagcggccgcGCTCCCTTGTTTAATAGCTT |
| Promotor Rev | aaaaggatccGCTTAACGGCAACTCAAAG |
| Terminator Fw | aaaactgcagTATCGTTTCCACTTTTTTCTG |
| Terminator Rev | aaaagcttGACCGGTTTTGCAAAGGACG |
| Tgl3 HA (N-term) Fw | aaaaggatccATGtacccatacgatgttcctgactatgcgAAGGAAACGGCGCAGG |
| Tgl3 HA (C-term) Rev | aaaactgcagCTAcgcatagtcaggaacatcgtatgggtaCCTACTCCGTCTTGCTCTTA |
| Tgl3 MYC (N-term) Fw | aaaaggatccATGgaacaaaagctaatctccgaggaagacttgAAGGAAACGGCGCAGG |
| Tgl3 Fw | aaaaggatccATGAAGGAAACGGCGCAGG |
| Tgl3 Rev | aaaactgcagCTACCTACTCCGTCTTGCTCTTA |
| 1-kDa truncation (C-term) | aaaactgcagctaTAATTTAAATTCGACTGCAC |
| 2.4-kDa truncation (C-term) | aaaactgcagctaAATTAAAGCTAGTGCGGGCC |
| 5-kDa truncation (C-term) | aaaactgcagctaACCTTCAATAATCCTTGTTAATG |
| 1-kDa truncation (N-term) | aaaaggatccatgGTGTCTGCTGTAATACCGACC |
| 2-kDa truncation (N-term) | aaaaggatccatgAAAAACTGGATACTGCGTGTAG |
| 5-kDa truncation (N-term) | aaaaggatccatgATCACCGATATTTATTTCTTC |
| Deletion of RARRSR Rev | ttttctgcagctaTATTATGTCGTCTAATTTAAA |
| Deletion of FKLDDII Fw | aagacaagatgtgcagtcgaaAGAGCAAGACGGAGTAG |
| Deletion of FKLDDII Rev | ctactccgtcttgctctTTCGACTGCACATCTTGTC |
| Exchange of F630A Fw | GATGTGCAGTCGAAgcTAAATTAGACGAC |
| Exchange of F630A Rev | GTCGTCTAATTTAgcTTCGACTGCACATC |
| Exchange of L632A Fw | GTGCAGTCGAATTTAAAgcAGACGACATAATAAGAGC |
| Exchange of L632A Rev | GCTCTTATTATGTCGTCTgcTTTAAATTCGACTGCAC |
| Exchange of D633A Fw | TGTGCAGTCGAATTTAAATTAGcCGACATAATAAGAGCAAGACGGAGT |
| Exchange of D633A Rev | ACTCCGTCTTGCTCTTATTATGTCGgCTAATTTAAATTCGACTGCACA |
| Exchange of D634A Fw | TGTGCAGTCGAATTTAAATTAGACGcCATAATAAGAGCAAGACGGAGT |
| Exchange of D634A Rev | ACTCCGTCTTGCTCTTATTATGgCGTCTAATTTAAATTCGACTGCACA |
| Exchange of D633A/D634A Fw | TGTGCAGTCGAATTTAAATTAGcCGcCATAATAAGAGCAAGACGGAGT |
| Exchange of D633A/D634A Fw | ACTCCGTCTTGCTCTTATTATGgCGgCTAATTTAAATTCGACTGCACA |
| Exchange of I635A/I636A Fw | GTCGAATTTAAATTAGACGACgcagcaAGAGCAAGACGGAGTAGGTAG |
| Exchange of I635A/I636A Rev | CTACCTACTCCGTCTTGCTCTtgctgcGTCGTCTAATTTAAATTCGAC |