TABLE 4.
Plasmids used in this study
aa, amino acids.
| Name | Relevant information | Source |
|---|---|---|
| pRS315 | CEN, LEU2, | 32 |
| pRS315-MYC-TGL3-HA | CEN, LEU2, | This study |
| pRS315-HA-TGL3 | CEN, LEU2 | This study |
| pRS315-HA-tgl3-1kD | CEN, LEU2, TGL3 is N-term HA-tagged and C-term-truncated (last 9 aa missing) | This study |
| pRS315-HA-tgl3-2.4kD | CEN, LEU2, TGL3 is N-term HA-tagged and C-term-truncated (last 20 aa missing) | This study |
| pRS315-HA-tgl3-5kD | CEN, LEU2, TGL3 is N-term HA-tagged and C-term-truncated (last 43 aa missing) | This study |
| pRS315-TGL3-HA | CEN, LEU2 | This study |
| pRS315-1kD-tgl3-HA | CEN, LEU2 TGL3 is C-term HA-tagged and N-term truncated (first 9 aa missing) | This study |
| pRS315-2kD-tgl3-HA | CEN, LEU2, TGL3 is C-term HA-tagged and N-term truncated (first 18 aa missing) | This study |
| pRS315-5kD-tgl3-HA | CEN, LEU2, TGL3 is C-term HA-tagged and N-term truncated (first 43 aa missing) | This study |
| pRS315-HA-tgl3-RARRSR | CEN, LEU2, TGL3 is N-term HA-tagged and the last 6 aa (RARRSR) are missing | This study |
| pSR315-HA-tgl3-FKLDDII | CEN, LEU2, TGL3 is N-term HA-tagged and the aa 630-636 (FKLDDII) are missing | This study |
| pRS315-HA-tgl3-DDAA | CEN, LEU2, TGL3 is N-term HA-tagged and aa Asp-633 Asp-634 are both replaced by A | This study |
| pRS315-HA-tgl3-DDEE | CEN, LEU2, TGL3 is N-term HA-tagged and aa Asp-633 Asp-633 are both replaced by E | This study |
| pRS315-HA-tgl3-D633A | CEN, LEU2, TGL3 is N-term HA-tagged and aa Asp-633 is replaced by Ala | This study |
| pRS315-HA-tgl3-D634A | CEN, LEU2, TGL3 is N-term HA-tagged and aa Asp-634 is replaced by Ala | This study |
| pRS315-HA-tgl3-IIAA | CEN, LEU2, TGL3 is N-term HA-tagged and aa Ile-635 Ile-636 are both replaced by Ala | This study |
| pRS315-HA-tgl3-F630A | CEN, LEU2, TGL3 is N-term HA-tagged and aa Phe-630 is replaced by Ala | This study |
| pRS315-HA-tgl3-L632A | CEN, LEU2, TGL3 is N-term HA-tagged and aa Leu-632 is replaced by Ala | This study |
| pRS315-TGL3-6xHA | CEN, LEU2 TGL3 is C-Term HA6-tagged | This study |