FIGURE 7.
Rotary potential. a, rotary potential of the F1 catalytic waiting state. Probability densities of angular positions during the pause from the five molecules were transformed into rotary potentials according to Boltzmann's law: wild-type F1 (red), F1(βE190A) (blue), F1(βK164A) (green), and F1(αR364A) (orange). Determined potentials were fitted with the harmonic function ΔG = 1/2·κ ·θ2, where κ is the torsion stiffness. Determined stiffness values were 79, 42, 22, and 38 pN·nm for wild-type F1, F1(αR364A), F1(βK164A), and F1(βE190A), respectively. b, rotary torque plotted against rotary potential stiffness.