TABLE 2.
Emax % and logEC50 values of the (R,R′)-aminoFen- and ISO-induced cAMP responses in HEK cell lines stably expressing β2-AR WT or β2-AR Y308F
Calculations of logEC50 and Emax values were based on concentration-response profiles for the compound-stimulated cAMP production. Emax values are expressed as percentages of the Emax response of the β2-AR WT −PTX group. Means ± S.E., n = 3. *, p < 0.05; **, p < 0.01 versus −PTX group.
| Cell lines | (R,R′)-AminoFen |
ISO |
||||||
|---|---|---|---|---|---|---|---|---|
| −PTX |
+PTX |
−PTX |
+PTX |
|||||
| Emax % | log EC50 | Emax % | log EC50 | Emax % | log EC50 | Emax % | log EC50 | |
| β2-AR WT | 100 ± 3 | −8.71 ± 0.05 | 101 ± 2 | −8.87 ± 0.03 | 100 ± 4 | −10.14 ± 0.10 | 97 ± 3 | −10.25 ± 0.07 |
| β2-AR Y308F | 89 ± 1 | −8.05 ± 0.01 | 117 ± 2** | −8.03 ± 0.02 | 109 ± 4 | −9.36 ± 0.06 | 126 ± 5* | −9.33 ± 0.07 |