FIGURE 1.
Upper panel, operon encoding P. furiosus MBH. The Mrp module is encoded by MbhA–H, and the hydrogenase module is encoded by MbhI–N. Lower panel, schematic representation of P. furiosus MBH. The enzyme is predicted to contain three [4Fe-4S] clusters in addition to the [NiFe]-catalytic site. The His9 affinity tag was attached to either MbhJ or MbhN as indicated. Fdred and Fdox represent the reduced and oxidized forms of P. furiosus ferredoxin. The predicted size (kDa) of each MBH subunit is shown in the table.