Skip to main content
. 2014 May 23;289(28):19364–19372. doi: 10.1074/jbc.M114.567255

FIGURE 2.

FIGURE 2.

The genetic strategy used to insert the His9 tag into MBH. A, the tag is inserted at the C terminus of mbhN (yielding P. furiosus strain MW0403) where PF1437 is the gene immediately downstream of the MBH operon. B, the tag is inserted at the N terminus of mbhJ (yielding P. furiosus strain MW0414). UFR, upstream flanking region (1 kb) of the MBH operon; DFR, downstream flanking region (1 kb) of the MBH operon; pyrF, selectable marker; Pgdh and Pslp, promoters for the gene encoding glutamate dehydrogenase and the S-layer protein of P. furiosus, respectively. The affinity tag was a His9 tag.