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. 2014 May 21;289(28):19395–19407. doi: 10.1074/jbc.M114.575142

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Purification and characterization of in vitro synthesized CPSA. A, in vitro synthesized CPSA (CPSA_iv) was separated from all contaminating reaction products using anion exchange chromatography with the indicated sodium chloride gradient. B, purified CPSA_iv after O-acetylation (CPSA_iv(OAc)) was re-purified under the same conditions resulting in a well separated product peak, which in panel C dot blot analyses were recognized by mAb 932. D, corresponding ¹H NMR analysis of the produced CPSA_iv in comparison to CPSA_n (from natural source). Slight variations in chemical shifts between CPSA_n and CPSA_iv are due to pH variations resulting from different purification protocols.