FIGURE 7.

Calpain-mediated disassembly of intercalated disc proteins in rat neonatal cardiomyocytes. A, immunofluorescence of N-cadherin and β-catenin in rat neonatal cardiomyocytes. Cells were pretreated with MDL28170 (MDL, 10 μm) and stimulated with ionomycin (Iono, 10 μm) for 10 min. N-cadherin and β-catenin are represented in green and red, respectively. Experiments were repeated three times in triplicate, and the representative images are shown. Scale bars, 10 μm. B, immunofluorescence of N-cadherin and Cx43 in rat neonatal cardiomyocytes. Cells were pretreated with MDL28170 (10 μm) and stimulated with ionomycin (10 μm) for 10 min. N-cadherin and Cx43 are represented in green and red, respectively. Experiments were repeated three times in triplicate, and the representative images are shown. Scale bars, 10 μm.