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Analysis of embryonic skin keratinocytes in primary culture by indirect immunofluorescence. Conventional fluorescence microscopy of keratinocytes harvested from Krt5+/+ (Control; A–A″), Krt5−/− (Krt5 null; B–B″), or KRT8bcTg/−Krt5−/− (KRT8bc Repl.; C–C″) E18.5 embryos (as indicated on top) is shown. Fixed cells were immunostained for keratin 5, keratin 8, and keratin 14 epitopes as indicated at left. Nu, nucleus. Bars, 10 μm.
