FIGURE 1.

STX8 physically interacts with TrkA but not TrkB receptors. A, co-immunoprecipitation of STX8-HA with FLAG-Trk receptors. Lysates from HEK293 cells transfected with STX8-HA and FLAG-TrkA/TrkB constructs were immunoprecipitated with anti-FLAG antibody. Immunoblotting (IB) analysis was performed to detect immunoprecipitated proteins. B, endogenous association of STX8 and Trk receptors. Tissue homogenates from adult rat DRG were immunoprecipitated with the STX8 antibody and immunoblotted with Trk antibodies. C, immunohistochemical analysis was performed with DRG sections labeled with anti-TrkA (green) and anti-STX8 (red) antibodies. Scale bar, 20 μm. D, co-localization of endogenous TrkA and STX8 in cultured DRG neurons visualized by confocal microscopy. The lower panels show enlarged images of the framed regions. The arrows in the combined image indicate punctate staining of co-localized TrkA and STX8. Scale bar, 5 μm.