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. 2014 May 28;289(28):19627–19636. doi: 10.1074/jbc.M113.501148

FIGURE 1.

FIGURE 1.

Reactivity and specificity of anti-phospho-Tyr-15 Cdk5 antibodies. A, Cdk5 or Cdk5 Y15F was cotransfected into COS-7 cells with ca Fyn. Cell lysates were immunoblotted with anti-phospho-Tyr-15 Cdk5 (ab) or (sc), anti-phospho-Tyr-15 Cdk1 (cs), anti-Cdk5 C8, and anti-Fyn. B, anti-phospho-Tyr-15 Cdk5 antibody does not react with Cdk1 and Cdk2. caFyn was transfected in COS-7 cells with or without Cdk5. Cell lysates were immunoblotted with anti-Cdk5 C8, anti-Cdk1, anti-Cdk2, anti-phospho-Tyr-15 Cdk5 (sc), and anti-phospho-Tyr-15 Cdk1 (cs).