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p97 regulates the interaction between HERC2 and USP33. A, co-immunoprecipitation of HERC2 and USP33 upon p97 inhibition. HeLa cells were treated with control (DMSO) or 10 μm NMS-873 for 12 h, and total lysates were harvested for immunoprecipitation (IP) using an anti-HERC2 antibody. Co-immunoprecipitated proteins were detected by immunoblotting. B, similar to A, except anti-USP33 antibodies were used to immunoprecipitate USP33.
