FIGURE 3.

Heat modifiability of TpsB2 and mutant derivatives of TpsB2. Immunoblot of outer membrane preparations of N. meningitidis HB-1 tpsB1::kan/tpsB2::gen cells expressing the TpsB variants indicated above the lanes. Samples were either heated for 10 min at 100 °C (D) or kept at room temperature (N). The blot was incubated with anti-TpsB2. The positions of the bands representing folded TpsBs are indicated on the left side, and the molecular weight markers are shown on the right. The panels were taken from the same blot. The TpsB2_ExP1P2B1 was co-expressed with either the TPS2a or the TPS1 construct, as indicated.