TABLE 1.

Primers and cloning strategies used for tpsB ORFs

^a Primer sequences are given when the primer is mentioned for the first time; restriction sites used for cloning are underlined.

^b PCR1 and PCR2 indicate the use of an amplicon of a PCR from a previous step in the cloning procedure.

^c Chromosomal DNA came from cell lysates of N. meningitidis strain H44/76; the ORFs mentioned are inserted in pGEM-T vectors; the amplicons mentioned derive from previous steps in the cloning procedure.