TABLE 2.
Expression and cloning vectors used in this study
| Construct | Plasmid name | TPS ORFa | Source/Reference |
|---|---|---|---|
| TPS2a with TpsB2 and its derivatives | |||
| TPS2a + TpsB2 | pEN1220 | tpsA2a-tr | Ref. 26 |
| TPS2a + TpsB2_ΔP1 | pEN1222 | tpsA2a-tr | This study |
| TPS2a + TpsB2_ΔP2 | pEN1223 | tpsA2a-tr | |
| TPS2a + TpsB2_ΔP1P2 | pEN1224 | tpsA2a-tr | |
| TPS2a + TpsB2_P1P1 | pEN1226 | tpsA2a-tr | |
| TPS2a + TpsB2_P2P2 | pEN1227 | tpsA2a-tr | |
| TPS2a + TpsB2_ExP1P2B1 | pEN1225 | tpsA2a-tr | |
| TPS2a + TpsB2_ExP1B1 | pEN1232 | tpsA2a-tr | |
| TPS2a + TpsB2_ExP2B1 | pEN1233 | tpsA2a-tr | |
| TPS2a + TpsB2_RevP2P1 | pEN1234 | tpsA2a-tr | |
| TPS2a with TpsB1 and its derivatives | |||
| TPS2a + TpsB1 | pEN1250 | tpsA2a-tr | Ref. 26 |
| TPS2a + TpsB1_ExP1P2B2 | pEN1229 | tpsA2a-tr | This study |
| TPS2b with TpsB2 and its derivatives | |||
| TPS2b + TpsB2 | pEN1320 | tpsA2b-tr | Ref. 26 |
| TPS2b + TpsB2P2P1 | pEN1334 | tpsA2b-tr | This study |
| TPS1 with TpsB1, TpsB2, and their derivatives | |||
| TPS1 + TpsB1 | pEN1030 | tpsA1a-tr | Ref. 26 |
| TPS1 + TpsB2 | pEN1050 | tpsA1a-tr | Ref. 26 |
| TPS1 + TpsB1_ExP1P2B2 | pEN1029 | tpsA1a-tr | This study |
| TPS1 + TpsB2_ExP1P2B1 | pEN1028 | tpsA1a-tr | |
| TPS1 + TpsB2_ExP1B1 | pEN1032 | tpsA1a-tr | |
| TPS1 + TpsB2_ExP2B1 | pEN1033 | tpsA1a-tr | |
| TPS1 + TpsB2_RevP2P1 | pEN1034 | tpsA2b-tr | |
| Not combined with TPS construct | |||
| TpsB2_ExP1P2B1 | pEN_tpsB2_ExP1P2B1 | This study | |
| TpsB1_ExP1P2B2 | pEN_tpsB2_ExP1P2B2 | ||
| Cloning vectors | |||
| TPS1 | pPU1000 | tpsA1a-tr | Ref. 19 |
| TPS2a | pPU1200 | tpsA2a-tr | Ref. 19 |
| TPS2b | pPU1300 | tpsA2b-tr | Ref. 19 |
| pGEM-T | Finnzymes | ||
| pEN300 | Ref. 27 | ||
a The suffix “-tr” indicates that the construct comprises a truncated tpsA ORF that encodes the signal peptide and the TPS domain (26).