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. 2009 Nov 12;20(12):1607–1626. doi: 10.1089/hum.2009.109

FIG. 7.

FIG. 7.

Comparison of engraftment levels of human CD34+ progenitor cells in NSG mice. At the indicated times after neonatal transplantation of human CD34+ cells, human CD45+ cells in mouse peripheral blood (PB) were assayed by flow cytometric analysis. Freshly isolated human CD34+ cells isolated from CB were coelectroporated with 10 μg of pT/MNDU3-eGFP-BGH and 1 μg of pEF1-α-HSB plasmid (group C; n = 11) or with 10 μg of pT/EF1-α-eGFP-BGH and 1 μg of pEF1-α-HSB plasmid (group D; n = 11). As study controls, NSG mice received human CD34+ cells that were not transduced (group A; n = 3) or that were “mock transduced” (electroporated without plasmid DNA) (group B; n = 4). Asterisks (* and **) indicate significant differences (p < 0.01) between the data points marked.