Table 2.

Comparison of Stable Transgene Expression Achieved with HSB Transposase Expressed by NMDU3, CMV, or EF1-α Promoter in K-562, Jurkat, and Primary Human CD34⁺ Cells

Cell type	CMV-HSB-mediated gene expression (% eGFP ± SEM)	MNDU3-HSB-mediated gene expression (% eGFP ± SEM)	EF1-α-HSB-mediated gene expression (% eGFP ± SEM)	Fold increase
K-562 (n = 2)	76.0 ± 1.1	68.1 ± 13.6	85.6 ± 0.7	1.1 (p = 0.18)
Jurkat (n = 2)	8.0 ± 2.1	7.6 ± 1.6	16.6 ± 2.3	2.1 (p = 0.006)
LTC-CD34+ (n = 3)	3.8 ± 0.8	2.1 ± 1.2	6.9 ± 1.8	1.8 (p = 0.05)

Abbreviations: CMV, cytomegalovirus; EF1-α, elongation factor 1α; MNDU3, modified myeloproliferative sarcoma virus long terminal repeat enhancer–promoter.