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. Author manuscript; available in PMC: 2014 Oct 10.
Published in final edited form as: Cell. 2013 Oct 10;155(2):357–368. doi: 10.1016/j.cell.2013.09.008

Fig. 1. Troy is expressed in chief and parietal cells at the base of corpus glands.

Fig. 1

(A) Confocal image showing Troy-eGFP expression at the base of corpus glands in a Troy-ki mouse. Projection of six 1µm spaced z-stacks. (B) Troy-eGFP is expressed at gland bottoms throughout the corpus. Black arrows point to the base of the epithelial lining, white arrows to the muscle layer. (C–E) Confocal microscopy reveals that Troy-eGFP+ cells are co-expressing either the parietal cell marker H+K+-ATPase (C) or the chief cell marker gastric intrinsic factor (D). Basal enteroendocrine cells marked by chromogranin A are Troy-eGFP− (E). (F) Electron microscopy of cryo immunogold labeled Troy-eGFP+ cells. 15 nm Gold label corresponding to eGFP, visible as black dots. Both chief and parietal cells at the gland base express eGFP and positive cells possess characteristics of maturation specific to that lineage. See also Fig. S1.