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. 2013 Jul 31;89(3):72. doi: 10.1095/biolreprod.113.110981

FIG. 7.

FIG. 7

Fluorescence staining of four-cell rhesus embryos from XXO-treated sperm treatment group stained for α-tubulin (monoclonal anti-α-tubulin-FITC antibody) and Hoescht 33342 nuclear stain. a) Hoffman modulation contrast image of a four-cell embryo. b) Fluorescence image of same embryo shown in a. Note severe cellular fragmentation of DNA-containing fragmented cells; two blastomeres appear normal (arrows). c) Hoffman modulation contrast image of a four-cell fragmenting embryo demonstrating cellular debris and uneven-sized blastomeres. d) Fluorescence image of same embryo demonstrating several blastomeres without detectable DNA, fragmenting nuclei (arrow), and cell fragments containing DNA. Original magnification ×400.